DI THIZONE AR

DI THIZONE AR

SKU: RXSOL-60-6604-496
Category: Lab Chemicals

Dithizone is a sulfur-containing organic compound. It is a good ligand, and forms complexes with many metals such as lead and mercury. Dithizone may be prepared by reacting phenylhydrazine with carbon disulfide, followed by reaction with potassium hydroxide. Dithizone is used to assess the purity of human pancreatic islet preparations used for…

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Dithizone is a sulfur-containing organic compound. It is a good ligand, and forms complexes with many metals such as lead and mercury. Dithizone may be prepared by reacting phenylhydrazine with carbon disulfide, followed by reaction with potassium hydroxide.
 
Dithizone is used to assess the purity of human pancreatic islet preparations used for transplantation into patients with type 1 diabetes. Dithizone binds zinc ions present in the islet's beta cells, and therefore stains the islets red. Exocrine tissue also present in the preparations does not bind dithizone, and is therefore not stained.Dithizone is a sulfur-containing organic compound. It is a good ligand, and forms complexes with many metals such as lead and mercury. Dithizone may be prepared by reacting phenylhydrazine with carbon disulfide, followed by reaction with potassium hydroxide.
 
Dithizone is used to assess the purity of human pancreatic islet preparations used for transplantation into patients with type 1 diabetes. Dithizone binds zinc ions present in the islet's beta cells, and therefore stains the islets red. Exocrine tissue also present in the preparations does not bind dithizone, and is therefore not stained.


A precipitate is formed immediately upon addition of the carbon disulfide; the mixture becomes warm, and the temperature soon approaches the boiling point. The temperature is maintained just below the boiling point; cooling with ice water may be necessary to keep it there. As the carbon disulfide is added, the mixture soon becomes pasty, then more fluid, and finally pasty again.


Indicator for metals.
Dithizone has been used in the preparation of dithizone stain solution for pancreatic islet cells.

Dithizone (Diphenylthiocarbazone) may be used in the following studies:

  • In the cloud point preconcentration step, before the trace level quantification of silver by flame atomic absorption spectrometry.
  • Preparation of hexadecyltrimethyl ammonium bromide (CTAB) capped gold nanoparticles (AuNPs). This reagent was useful for the colorimetric detection of heavy metal ions.
  • As a zinc chelating ligand for the extraction of zinc in bovine serum.

A lethal dose of cadmium is 2 mg per 1 kg of body weight.


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